Misfolding-assisted Selection of Stable Protein Variants Using Phage Displays
نویسندگان
چکیده
منابع مشابه
Generating thermal stable variants of protein domains through phage display.
Often in protein design research, one desires to generate thermally stable variants of a protein or domain. One route to identifying mutations that yield domains that remain folded and active at a higher temperature is through the use of directed evolution. A library of protein domain variants can be generated by mutagenic PCR, expressed on the surface of bacteriophage M13, and subjected to hea...
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ژورنال
عنوان ژورنال: BMB Reports
سال: 2006
ISSN: 1976-6696
DOI: 10.5483/bmbrep.2006.39.1.055